6 Reasons to Check Your Substrate
- Sensitivity and cost per test - A sensitive substrate could allow you to achieve a better dilution on costly primary antibodies. If your staining is obscuring everything, a lighter substrate might offer better balance.
- Endogenous Enzymes - Struggling to block enzyme rich tissues? A different reporting enzyme and substrate could help clean up.
- Colour - Is it easy to see on your sample? A different colour to brown might be easier to see on pigmented tissue.
- Safety - DAB is a suspected carcinogen. Although it is not known if other substrates are carcinogenic too, liquid formulations are safer to use than tablets or powders.
- Consistency - Manufactured kits are tested against previous batches to ensure consistency and reliabiltiy.
- Working solution shelf life - Substrates are always best made up fresh, but if you're throwing working solution away, a different formulation may help minimize wastage.
All Vector Laboratories' substrates are provided in liquid format to minimise exposure and enable run appropriate volumes to be made for bench top runs, or on an autostasiner for suitable substrates. Wait times for mixing and dissolving powders or tablets are eliminated.
Kits make up the indicated volumes of working reagent, or can be manufactured in bulk quantities if required.