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Immunohistochemistry Workflow

Since 1976, Vector Laboratories has been at the forefront of developing innovative laboratory detection reagents. The VECTASTAIN® ABC systems established us as the leader in immunohistochemistry (IHC) and immunocytochemistry (ICC), and we remain a trusted name in these fields. Our products are well regarded for their high sensitivity, low background, reliability, reproducibility, and value pricing. We offer many different detection systems to accommodate the wide range of experimental priorities.

Selecting the appropriate reagents for your IHC assay is an important part of optimizing your protocol. Vector Laboratories is your resource for premium labeling and detection products at each step of your IHC workflow.

Tissue Preparation

VECTABOND™ Tissue Section Adhesive

ImmPrint™ Histology Pen

ImmEdge™ Hydrophobic Barrier Pen

Antigen Retrieval 

Antigen Unmasking Solution, Tris-Based

Antigen Unmasking Solution, Citric Acid Based

Quench / Block

BLOXALL™ Endogenous HRP and AP Blocking Solution

Avidin/Biotin Blocking Kit

Streptavidin / Biotin Blocking Kit

Normal Sera

Animal-Free Block and Diluent

BSA

Casein Solution

Vector® M.O.M.™ Mouse Ig Blocking Reagent

Carbo-Free Block

Primary Antibody / Lectins

Biotinylated lectins

Unconjugated lectins

Secondary Antibody

ImmPRESS™ Polymer Reagents (HRP or AP)

Mouse on Mouse (M.O.M.™) ImmPRESS™ Polymer Kit

Mouse on Mouse (M.O.M.™) Basic Kit

Biotinylated secondary antibodies

Unconjugated secondary antibodies

Enzyme-conjugated secondary antibodies (HRP or AP)

Biotinylated anti-lectins

Tertiary Reagent

VECTASTAIN® ABC complex reagents

ImmPRESS™ Excel Amplified Staining Kits

Mouse on Mouse (M.O.M.™) Elite® Kit (HRP)

Enzyme-conjugated avidin/streptavidin (HRP or AP)

Substrate / Chromogen

HRP substrates

AP substrates/ Levamisole Solution

Counterstain

Hematoxylin

Methyl Green

Nuclear Fast Red

Coverslip / Mount

VectaMount™ Mounting Medium

VectaMount™ AQ Mounting Medium

Visualise

 

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