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IHC Antibodies & Kits

Trusted Quality & Easy Handling

Since 1983, PROGEN is an established antibody manufacturer with a broad portfolio of >400 IHC antibodies used for research and diagnostics of about 40 pathologies in 25 different organs/tissues. The DIN ISO 13485 certified company provides commonly used monoclonal and polyclonal antibodies for diagnostics, prognostics and the analysis of predictive biomarkers. Some of which represent the most common antibody clones used pathology community.

Immunohistochemistry (IHC) was invented in the 1940s and has developed to be a valuable tool for the identification of abnormal cells and cancerous tissues by using specific molecular markers. Additionally, IHC represents a excellent tool to analyze the distribution and localization of biomarkers and differentially expressed proteins in context of the specific biological tissue.

Trusted pathology markers

PROGEN´s tissue- & tumor -specific antibodies (see table) are widely used pathology markers for basic research and clinical diagnostics of a variety of cancers. Most of the antibody clones are well-established, highly published and robust tools in health care and pathology. To serve today´s urgent customer need for high quality and reliablility, al

The monoclonal antibody clones against keratin 20 (Ks20.8),p53 (Bp53.11),EP-CAM (HEA125) and vimentin (Vim3B4)are only a few examples for approved clones that have been well trusted in the area of pathology for many years.

Table: Selection of PROGEN´s tissue- and tumor-specific antibodies

Organ / Tissue Pathology / Target Tissue Antibodies
Breast Breast Cancer / Lesion α-smooth muscle actin𝛿-catenin, cyclinD, p53, K5/8, K7, K8, K17, MUC1, MUC2, MUC6E-cadherin, p120 (catenin)Progesterone receptor
Mesenchyme Sarcoma α-smooth muscle actin, vimentin
Skin Skin cancer / Lesion EP-CAM, K19, adipophilin (PLIN2), neurofilament, CD11a, CD15, CD27, CD31CD57,  collagen type IV, myogenin, S-100
Eptihelial Marker K7, K8, K18, K20
Ovary Ovarian Cancer K7, vimentin, CA-125
Cervix Cervical Cancer / Neoplasia K7, K17, MUC1CD8a, CD27, Cdk1, S100A9
Endometrium Endometrium Cancer K7, MUC1, CD8aCD11a, CD27, estrogen eceptorProgesterone receptor
Endothelium Endothelial Cancer vimentin
Stomach Gastric Cancer K7, p216
Colon Colon Cancer K7, K8, K20MUC2
Kidney Kidney Cancer K7, K19K8, K18
Liver Liver Cancer K8K18, K19, TTF-1

prohisto IHC Staining Kits

Immunohistochemistry (IHC) is a well-established and very effective microscopy-based method that enables the visualization of cellular components (e.g. proteins or macromolecules) in cells and tissue samples. The method can detect and localize cellular components as well as provide information about their biological state. IHC is frequently used in health care and pathology as a powerful tool for clinical diagnostics and medical therapy.

In basic research, IHC plays an important role in the analysis of proteins and the elucidation of their role in health & disease. Since the invention of IHC in the 1940s, the range of applications has been steadily growing due to an increasing availability of specific antibodies against diverse targets and advanced detection technologies.

While IHC is a robust technique, the establishment of an IHC assay requires precise and often timeconsuming optimization since it depends on several factors, such as target molecule characteristics, choice of primary antibody, and analyzed tissue or cells. These factors play an important role in the production of reliable and reproducible data and are often underestimated.

With the prohisto IHC staining kits, PROGEN offers a product line that supplements its comprehensive portfolio of primary antibodies and features premium performance along with care-free handling – without the need for cumbersome optimization. The prohisto portfolio includes synchronized kits for secondary antibody detection (prohisto-HiSec) as well as for the visualization with DAB substrate (prohisto-HiVis). All products are carefully optimized for IHC, immunocytochemistry (ICC) and in-situ hybridization (ISH).

prohisto-HiSec prohisto-HiVis
FEATURES
The prohisto-HiSec products are secondary antibody kits for IHC containing a suitable blocking solution as well as the HRP-coupled secondary antibody for detection. They are available either as anti-guinea pig, anti-mouse or anti-rabbit detection kit. The prohisto-HiVis kit contains the chemical substrate DAB (3,3´diaminobenzidine) for the visualization of the detected target molecule.
BENEFITS
highly sensitive highly sensitive
ready-to-use easy handling, mix 1:1
polymerized peroxidase detection system heat-resistant
limited steric interference allows IHC/ISH double-labelling
enhanced target accessibility can be dehydrated, cleared & permanently mounted
efficient staining of nuclear & membrane antigens can be viewed by dark-field & electron microscopy

External validation for IHC applications

The prohisto product line has been externally validated for use with PROGEN´s primary IHC antibodies. IHC of human skin using rabbit anti-Histidine decarboxylase polyclonal serum (Cat. No. 16045, 1:5000) and prohisto-HiSec anti-rabbit HRP (Cat. No. PRSTAR).

PROGEN in cooperation with the University Hospital Heidelberg, J. Heß. IHC of human colon carcinoma using guinea pig antiKeratin K8/K18 antibody (Cat. No. GP11, 1:100) and prohisto-HiSec anti-guinea pig HRP (Cat. No. PRSTAG).

PROGEN IHC of rat colon using mouse anti-Keratin K20 (Cat. No. 61026, 1:200) and prohisto-HiSec anti-mouse HRP + prohisto-HiVis DAB duo set (Cat. No. PRDABM). © PROGEN in cooperation with the University Hospital Heidelberg, J. Heß.

Name SKU
prohisto-HiSec anti-guinea pig HRP PRSTAG
prohisto-HiSec anti-mouse HRP PRSTAM
prohisto-HiSec anti-rabbit HRP PRSTAR
prohisto-HiVis DAB duo PRDAB1
SETS
prohisto-Set anti-mouse HRP + DAB duo PRDABM
prohisto-Set anti-guinea pig HRP + DAB duo PRDABG
prohisto-Set anti-rabbit HRP + DAB duo PRDABR

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