Flow Cytometry Colour Chart

Flow cytometry introduction: What does flow cytometry tell us?

‘Pointillism’ is a technique in painting where small dots of colour are added by the artist, one-by-one, building up to form an image, maybe a man, or a lakeside scene like Seurat's Grande Jatte and Circus.

Data from flow cytometry is not dissimilar; it builds up an abstract picture of a sample, with each cell forming a kind of pointillist’s dot, its position on the painting represents some property of the cell, such as size, or ‘granularity’, its colour something like a fluorescent marker. As more sample, more cells, are analysed the picture builds up, cells with similar characteristics grouping together, into separate 'populations'.

But flow cytometry allows more than two properties to be measured; the picture becomes a sculpture, viewable from different angles, rather than just the front. For example, by using antibodies against different markers on a cell, each labelled with a different fluorescent dye, the picture becomes three, four, five or more, dimensional; revealing otherwise hidden details – like sub-pictures – behind each cluster of 2D dots.

Each new dimension reveals something different about a cell, the proteins it contains or the markers on its surface, for example. By selecting a population of cells – ‘gating’ – with similar size and granularity, say, smaller and smaller subsets of cells with specific physical properties can be isolated and identified.

It is this ability to drill down into samples, isolating and revealing distinct groups of cells with unique physical characteristics and properties that make flow cytometry such a powerful technique, used throughout the life sciences. Scientists in many different fields use flow cytometry assays for their analysis including cancer research, drug discovery, HIV screening, academic research, bioprocessing and more. To help meet this need we've created a diverse catalogue of products suited for use in flow cytometry, from whole assays to individual antibodies and labels.

 

Products

We've gathered together a wide range of flow cytometry products, from individual conjugated antibodies, to complete kits and microspheres for instrument set-up, calibration as well as practicle applications such as labelling. Our product manufacturers include AcrisBangs Laboratories, DendriticsDiacloneExbio, and Imgenex.


Antibodies

An important aspect of flow cytometry is choosing the right dye or conjugate for your specific situation, either to avoid spectral overlap, get the best signal to noise ratio, or simply to ensure that it's excitation wavelength is within the ranges possible for your flow cytometer. So if you're unsure about which Dye conjugate is best suited for your needs take a look at our Colour Chart and see if that helps.

If you already know which dyes your after, then click the link below to see the range of products we have available for it:

AF488 AP APC Azide free
Biotin Cy3 Cy5 Cy5.5
Cy7 Gold Dyomics 647 FITC
FITC-PE PE-Cy7 HRP Pacific Blue
PE-Cy5.5 PE PE-Cy5 TRITC
Texas Red      


Assay Kits

We have a number of ready to go flow cytometry assay kits, including:

Fix and Perm®  For suspension stainings and flow cytometric analyses of intracellular antigens.

KOMBITEST – A wide range of kits designed for the identification and enumeration of various cell populations, such as human mature NK cells in erythrocyte lysed whole blood (See figure 1 and 2, opposite)
 

Microspheres

Microspheres offer a huge diversity of different application for flow cytometry, from sample labelling and purification to calibration and diagnostics. Below are a some of our novel product line to assist in these applications.

SoluLink – The latest UV-tracable biotinylation and linkable magnetic beads

Nano-screenMag – A novel labelling and detection system now available in blue, green, orange, pink and red

Fluorescence Reference Standards – labelled with specific fluorochromes to exhibit the same spectral characteristics as labelled cells

Ultra Rainbow Fluorescent Particles –  internally labelled with multiple fluorophores, enabling excitation between 365-650nm and are used for general QC for 3-9 colour instruments

Quantum™ – for the detection threshold, resolution, and linearity of the instrument


Flow Cytometry Resources

Our partners Bangs Laboratories have created a flow cytometry technical reference guide that you can find HERE. It is a useful resource to help you understand more about flow cytometry.

 

If you're looking for a deeper introduction to flow cytometry, then our pals from Innova Biosciences and Chromocyte have you covered, below is a youtube video of their free webinar 'An introduction to flow cytometry'.

 

While flow cytometry is a powerful and flexible technique, it can also be complicated to put together panels of different antibodies and labels, which is why we're happy to also point you towards Chromocyte,  a FREE comprehensive online resource for designing multi-parameter flow cytometry experiments and finding flow cytometry antibody & cell-based assay products and knowledge.

 

Interesting Facts on Flow Cytometry

A recent report on the expected trends in flow cytometry has predicted major changes to the field over the next ten years that include smaller and easier to use lasers with enhanced applications, improvements in associated IT to reduce manufacture costs and the development of new monoclonal antibody and molecular diagnostic tests to further enhance and simplify current assays.

 

Flow Cytometry Colour Chart 

There are many dye-conjugates available today for analyses. Sometimes we just need a little help to choose / clarify which ones are best suited for our research. We hope that the chart below is helpful to you, in some way, as an aid to deciding the best product to opt for. If you are still unsure which one to opt for, then please to not hesitate to contact us on info@2bscientific.com

 

This is a representative chart for some of the Conjugated Dye products available:

Visualisation   Dye Excitation Max. (nm) Emmision (nm)
  AP 280/295 400
  DyLight™ 405 400 405 - 420
  HRP 356/406 450
  Pacific Blue™ 410 405 - 455
  Biotin 350 500
  DyLight™ 488 493 488 - 518
  FITC 494 488 - 518
  Alexa-Fluor® 488 496 488 - 519
  Pacific Orange™ 400 551
  Cy™ 3 548 532 - 566
  TRITC 541 532 - 572
  PE 496 488 - 575
  DyLight™ 549 562 532 - 576
  PE-Texas Red® 496 532 - 615
  Texas Red®  589 561 - 615
  PE-DyLight™ 594  496 532 - 618
  DyLight™ 594  593 561 - 618
  APC 650 640 - 660
  PE-Cy™ 5  496 532 - 664
  Cy™ 5  646 640 - 664
  Alexa Fluor® 647  650 640 - 665
  PE-DyLight™ 649 649 532 - 674
  DyLight™ 649 649 640 - 674
  PerCP 482 561 - 675
  PE-Cy™ 5.5  496 532 - 692
  PerCP-Cy™ 5.5  482 561 - 692
  Alexa Fluor® 700  702 640 - 774
  PE-Cy™ 7  496 532 - 774
  APC-Cy™ 7 650 640 - 774
  APC-Alexa Fluor® 750 650 640 - 775
  PE-DyLight™ 750  496 532 - 778
  APC-DyLight™ 750  650 640 - 778
  DyLight™ 750  752 778
  DyLight™ 800 770 794


 * Please note this chart is just meant to be a general guide - details can vary dependent upon the specific use of the items listed above.

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