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Antibody Production Services

Polyclonal Antibody Production
Monoclonal Antibody Production
Antibody Engineering Service
Recombinant Antibody Expression
Hybridoma Sequencing
Antibody Purification
Antibody Conjugation

With partner facilities based in North America where the local climate is mild all year round allowing animals to be housed in “open air” facilities, our antibody services are ideal for good quality, robust products with satisfaction guaranteed. This is because the natural environment, along with constant attention to the health and comfort of the animals, leads to a better immune response and higher quality antibody production.

The advantages of using our services:

  • Custom antibodies produced at licensed facilities in the North America
  • Short protocols available for research antibody development
  • Longer custom antibody protocols available for large quantity needs
  • Antigen design including analysis of peptide sequence, hydrophobicity, secondary structure, and antigenicity
  • Fast, high quality peptide synthesis also performed within the United States
  • Certified animal technicians with over 20+ years of experience
  • All custom antibody development services are confidential
  • Newly constructed, state-of-the-art ascites production facility

Our Services:

  • Monoclonal Antibody Production Service
    • Hybridoma Generation - using automated high throughput automated platform that allows for more efficient clone testing at a very affordable price. This approach benefits from far less redundancy than the normal limit dilution method, ie, creating more diversified clones. Email us for more information
    • Recombinant Rabbit Monclonals - Our partner labs use molecular engineering  to provide rabbit monoclonal antibodies without the use of a fusion partner. Thus, a very practical way to benefit from the rabbit’s immune system. Email us for more information at Info@2BScientific.com
  • Polyclonal Antibody
    • Rabbit - with on-site veterinarians to continual monitor animal health & comfort, our facility is able to offer standard 60 day rabbit production packages which consist of 4 immunizations, and 3 production bleeds, typically using 2 New Zealand White Rabbits for each project. Protocols can be adjusted to suit specific needs. Email us for more information at Info@2BScientific.com
    • Goat - standard 98 day protocols, with 5 immunisations and 3 bleeds per goat per project. Email us for more information at Info@2BScientific.com
    • Chicken - Standard 60 protocol at the conclusion of which  0.5 gram purified (>90% pure) IgY are provided.  along with a pre-immune IgY sample. This is designed to provide a quick supply of sufficient custom chicken antibody to carry out research project. An immunisation/production schedule is provided for every project.
      • See our selection of Anti-Chicken Immunoglobulin Secondary Reagents
        Hens are housed and eggs are collected for an additional 2 weeks following delivery. At this time, the customer may choose to purchase more IgY. Email us for more information at Info@2BScientific.com
  • Antibody Engineering Service - using a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Our partner antibody engineering services include chimerisation, humanisation, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies. Email us for more information at Info@2BScientific.com
  • Recombinant Antibody Expression - to meet the growing demands for recombinant antibodies across research and diagnostics our partner lab have launched their proprietary transient expression system for rapid manufacturing of milligram to gram quantities of antibody in mammalian HEK293 cells. A faster more affordable alternative to stable CHO cell line generation with higher quality and flexibility than traditional hybridoma production. Simply provide us with an electronic sequence or hybridoma cell line and we’ll take care of the rest. We’ll even guarantee the quantity and quality of the antibody for all your in vitro or in vivo needs. Email us for more information at Info@2BScientific.com
  • Hybridoma Sequencing - quick and professional hybridoma sequencing service, also known as antibody sequencing. Hybridoma sequencing is the sequencing of the cDNA encoding the variable heavy (VH) and variable light (VL) domains of your hybridoma cell line. Email us for more information at Info@2BScientific.com
  • Antibody Purification - whether its monoclonal or polyclonal, a wide selection of sample types can be purified from ascites, sera to tissue culture supernatant ranging from a [For m few mg to multi-gram quantities. Email us for more information at Info@2BScientific.com
    • Ammonium sulphate precipitation
    • Protein A chromatography
    • Protein G chromatography
    • Antgien affinity purification
    • PEG Purification
  • Antibody Conjugation - the ability to conjugate most antibodies with:
    • HRP
    • Biotin
    • Fluorescent Dyes (FITC, Cy3, Cy5, Alexa Fluor etc.)

Email us for more information at Info@2BScientific.com

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