CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats. CRISPR sequences were originally identified in the Escherichia coli (E. coli) genome, and were found to function as part of an RNA-based adaptive immune system to target and destroy genetic parasites at the DNA level. CRISPR-associated protein (Cas) is an endonuclease that cuts foreign DNA, allowing integration into the host genome. Cleavage only occurs when there is a protospacer adjacent motif (PAM) around the targeted sequence of the invading DNA, ensuring highly accurate targeting.
Researchers studying CRISPR have adapted it for use as a tool for genetic modification of the target host genome. CRISPR/Cas9 has recently become a popular genome editing tool, due to its simplicity and versatility.
Cellecta CRISPR Products and Services: The CRISPR Cas9 system can be used for knocking out gene expression in vivo or in vitro by using a combination of an sgRNA (single guide RNA) along with the Cas9 nuclease. Achieve permanent 100% knockout in your cell line by using Cellecta’s lentiviral-based CRISPR constructs. Expression of the sgRNA and Cas9 is stable, and constructs can be used in dividing or non-dividing cells or whole model organisms. Let Cellecta design and make CRISPR sgRNA constructs for you or create a CRISPR sgRNA library for your system.
Grassian AR, Scales TM, Knutson SK, Kuntz KW, McCarthy NJ, Lowe CE, Moore JD, Copeland RA, Keilhack H, Smith JJ, Wickenden JA, Ribich S. A Medium-Throughput Single Cell CRISPR-Cas9 Assay to Assess Gene Essentiality. Biol Proced Online. 2015 Nov 14;17:15. eCollection 2015. PubMed PMID: 26578851; PubMed Central PMCID: PMC4647477.