Dyes in BOLDFACE are standard products available from Biosearch. These and the BHQ dyes are available in one or more of the following forms: labeled oligos, phosphoramidites, CPGs, pre-packaged DNA synthesis columns, carboxylicacids, peptide synthesis resins, succinimidyl esters, and amine labels.
*QR (Quenching Range) stands for each BHQ dye’s FRET quenchingrange. Fluorophore dyes are shown for informational purposes only.Non-Biosearch fluorophores listed may be trademarked by companiesother than Biosearch Technologies and may not necessarily be available
from Biosearch, please visit www.biosearchtech.com for full disclosure.
These recommendations apply to dual-labeled fluorescence-quenched probes. They should not be used as guidelines for TAMRA-quenched probes or for Hybridization Probes that rely on FRET as a means of excitation.
Some instruments will require fluorescence calibration. Fluorescence calibration allows these instruments to record the spectral profile for the dye(s) to be used in subsequent assays by resolving the total detected light into signals contributed by the individual fluorophores.
Recommendations highlighted in yellow have not been proven and should be used cautiously on an experimental basis. *SuperROX dye is a proprietary passive reference available from Biosearch Technologies.