- Flow Cytometry & Microscopy
Flow Cytometry & Microscopy
Flow cytometry uses the principles of light scattering , light excitation, and emission of fluorochrome molecules to generate specific multi-parameter data from particles and cells in the size range of 0.5um to 40um diameter. Cells are hydro-dynamically focused before intercepting an optimally focused light source. Lasers are most often used as a light source in flow cytometry. One unique feature of flow cytometry is that it measures fluorescence per cell or particle. This contrasts with spectrophotometry in which the percent absorption and transmission of specific wavelenths of light is measured for a bulk volume of sample.
Flow cytometry analysis of a single cell suspension yields multiparameter data corresponding to Forward Light Scatter (FLS), 90° Light Scatter (90LS), and FL1-FL4. This information allows researchers to identify and characterize various subpopulations of cells. The process of separating cells using flow cytometry multiparameter data, is referred to as sorting.
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Special Promotions |
BRAND NEW Pacific Orange™& Pacific Blue™ conjugates, together give the user a two colour scheme in which both are excited at 405nm and emit at 551nm and 455nm respectively. Both conjugates are also ideally suited for use with fluorescence microscopes |
| Discover the Great range of Conjugated Cell Surface Antibodies |
Resources
Unsure about which Dye conjugate is best suited for your needs? - take a look at our Colour Chart and see if that helps
Product
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BRAND NEW Pacific Orange™ & Pacific Blue™ conjugates, together give the user a two colour scheme in which both are excited at 405nm and emit at 551nm and 455nm respectively. Both conjugates are also ideally suited for use with fluorescence microscopes
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SoluLink – the Latest UV-traceable Biotinylation and Linkable Magnetic Beads
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Discover the Great range of Conjugated Cell Surface Antibodies
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KOMBITEST – HLA-B27 FITC / HLA-B7 PE designed for identification & enumeration of cell populations
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NIST traceable standards – mono-disperse polystyrene spheres available in diameters ranging from 40nm-175µm
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Label & Detect with the novel nano-screenMag – now available in Blue, Green, Orange, Pink & Red
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BasoFlowEx® K - for flow cytometry examination of IgE-mediated allergic reactions
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C-Phycocyanin (C-PC - Cyanobacterial Phycobiliprotein) – The Brand New Label with high fluorescence quantum yield, molar extinction coefficients, absorbtivity and stability that can be easily cross linked with antibodies & proteins with conventional molecular tagging
BRAND NEW Pacific Orange™ & Pacific Blue™ conjugates, together give the user a two colour scheme in which both are excited at 405nm and emit at 551nm and 455nm respectively. Both conjugates are also ideally suited for use with fluorescence microscopes
SoluLink – the Latest UV-traceable Biotinylation and Linkable Magnetic Beads
Discover the Great range of Conjugated Cell Surface Antibodies
KOMBITEST – HLA-B27 FITC / HLA-B7 PE designed for identification & enumeration of cell populations
NIST traceable standards – mono-disperse polystyrene spheres available in diameters ranging from 40nm-175µm
Label & Detect with the novel nano-screenMag – now available in Blue, Green, Orange, Pink & Red
BasoFlowEx® K - for flow cytometry examination of IgE-mediated allergic reactions
C-Phycocyanin (C-PC - Cyanobacterial Phycobiliprotein) – The Brand New Label with high fluorescence quantum yield, molar extinction coefficients, absorbtivity and stability that can be easily cross linked with antibodies & proteins with conventional molecular tagging
Chromocyte is a FREE comprehensive online resource for designing multi-parameter flow cytometry experiments and finding flow cytometry antibody & cell-based assay products and knowledge
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